Catalog Number:SRT0201
CAS:9002-07-7
EC:3.4.21.4
Storage temperature: 2-8℃
Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli
Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin
1.DESCRIPTION
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
2.MAIN FEATURES
Source | Recombinant E. coli |
Appearance | White or off white, or yellowish powder |
Specific activity | ≥4500 USP units/mg pro. |
SDS-PAGE | Single major band |
Molecular Weight(SDS-PAGE) | 24.0±2.4 kDa |
Purity(HPLC) | ≥95% |
3.RECOMMEND USAGE
It is recommended to dissolve or dilute with 50 mM HAc. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.
It is no activity loss after 5 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
1)Sequence grade recombinant protein: high specificity,good stability.
2)Animal origin free: sequencing grade modified trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
3)Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
4)High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
5)Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
6)Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory
requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
7)Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.
7.RELATED PRODUCTS
Recombinant enterokinase
Recombinant V8 protease
Sequencing grade chymotrypsin
Sequencing grade carboxypeptidase B
Product quality protection 
